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KMID : 0191119980130040395
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Journal of Korean Medical Science
1998 Volume.13 No. 4 p.395 ~ p.399
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Detection of t(11;22)(q24;q12) translocation of Ewing´s sarcoma in paraffin embedded tissue by nested reverse transcription-polymerase chain reaction
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Park YK
Chi SG/Park HR/Yang MH/Unni KK
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Abstract
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Ewing´s sarcoma is a poorly characterized malignant tumor with a relatively uniform histologic appearance,
made up of densely packed small cells with round to oval nuclei, without distinct cell borders and without
any structural differentiation. Often the diagnosis has to be made by exclusion. Recently, it has been made
possible to identify characteristic chromosomal rearrangements associated with certain solid tumors. More
than 85% of Ewing´s sarcoma and peripheral neuroectodermal tumor present a specific t(11;22)(q24;q12)
balanced translocation, resulting in the production of a novel chimerical EWS/FLI-1 message. Using
oligonucleotide primers derived from EWS and FLI-1 complementary DNAs, we were able to use reverse
transcription-polymerase chain reaction (RT-PCR) as a diagnostic tool. The described nested RT-PCR
method as another supportive diagnostic method enables pathologists to differentiate small blue cell
tumors not only to make correct diagnosis but also to investigate retrospective archival tumor samples,
using formalin fixed paraffin embedded tissue as a source of RNA.
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